Journal: Journal of orthopaedic surgery and research

Article Title: Hsa_circ_0001017 promotes cell proliferation, migration and invasion in osteosarcoma by sponging miR-145-5p.

doi: 10.1186/s13018-022-03062-z

Figure Lengend Snippet: Fig. 2 Validation of five circRNAs. Quantitative real-time PCR was used to determine the expression levels of hsa_circ_0000704, hsa_circ_0001016, hsa_circ_0001017, hsa_circ_0005035 and hsa_circ_0008934 in human OS cell lines (Saos-2, U2OS and MG63) and osteoblast cell lines (HECC, hFOB1.19 and HFF-1)

Article Snippet: The human OS cell lines (Saos-2, U2OS and MG63) and osteoblast cell lines (HECC, hFOB1.19 and HFF-1) were purchased from the American Type Culture Collection (USA).

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Expressing

Journal: Molecules

Article Title: Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells

doi: 10.3390/molecules25092238

Figure Lengend Snippet: Figure 3. IL-8 production by human foreskin fibroblasts line HFF-1 exposed to fractionated E. rhamnoides (L.) extracts and reference compounds: (a) 6 h; (b) 24 h, tested by ELISA. The results are given as a mean concentration [pg/mL] ± standard error. C1—control cells in culture medium with Me-OH, OF/GF/LF—phenolic fractions of fruit/twig/leaf extracts, C2—control cells in culture medium with DMSO, OL/GL/LL—non-polar fractions of fruit/twig/leaf extracts, PG—S. aureus peptidoglycan, TNF—murine tumor necrosis factor alpha, KU—ursolic acid, Q—quercetin, E—(−)-epicatechin, KE—ellagic acid; * p < 0.05, ** p < 0.01.

Article Snippet: Human epithelial colorectal adenocarcinoma cells (Caco-2, HTB-37) and human foreskin fibroblasts (HFF-1, SCRC-1041) were obtained from LGC Standards (Teddington, Middlesex, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Control

Journal: Antioxidants

Article Title: Anthocyanin-Related Pigments: Natural Allies for Skin Health Maintenance and Protection

doi: 10.3390/antiox10071038

Figure Lengend Snippet: Cell viability of HaCat and HFF-1 cell lines in the presence of increasing concentrations of the compounds, evaluated by MTT assay. Dotted lines represent 80% viability.

Article Snippet: Spontaneously transformed aneuploid immortal keratinocyte cell lines from adult human skin (HaCat) and human foreskin fibroblasts (HFF-1) were cultured in 22.1 cm 2 plates and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Cell Lines Service), supplemented with 10% or 15% fetal bovine serum (FBS, CLS) and 1% of antibiotic/antimycotic solution (100 units/mL of penicillin, 10 mg/mL of streptomycin and 0.25 mg/mL of amphotericin B, Sigma-Aldrich, St. Louis, MO, USA), at 37 °C in an atmosphere of 5% CO 2 .

Techniques: MTT Assay

Journal: Antioxidants

Article Title: Anthocyanin-Related Pigments: Natural Allies for Skin Health Maintenance and Protection

doi: 10.3390/antiox10071038

Figure Lengend Snippet: Effect of anthocyanins and related structures on ROS production in HaCat and HFF-1 cell lines, 24 h after incubation with 50 µM of each compound. Hydrogen peroxide (50 µM) was used as a positive control. Statistically significant differences between ROS production in the presence of the different compounds and control group are marked with asterisks: p < 0.05 (*), p < 0.005 (**) and p < 0.0001 (****). Dotted lines represent 80% ROS production.

Article Snippet: Spontaneously transformed aneuploid immortal keratinocyte cell lines from adult human skin (HaCat) and human foreskin fibroblasts (HFF-1) were cultured in 22.1 cm 2 plates and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Cell Lines Service), supplemented with 10% or 15% fetal bovine serum (FBS, CLS) and 1% of antibiotic/antimycotic solution (100 units/mL of penicillin, 10 mg/mL of streptomycin and 0.25 mg/mL of amphotericin B, Sigma-Aldrich, St. Louis, MO, USA), at 37 °C in an atmosphere of 5% CO 2 .

Techniques: Incubation, Positive Control

Journal: Pharmaceutics

Article Title: In Vitro Activity of Carbosilane Cationic Dendritic Molecules on Prevention and Treatment of Candida Albicans Biofilms

doi: 10.3390/pharmaceutics12100918

Figure Lengend Snippet: Cytotoxicity of antifungals (CSF and AmB), BDSQ024 and combinations on human cell lines. ( A ) CSF; ( B ) AmB; ( C ) BDSQ024 on HeLa cells; ( D ) BDSQ024 on HFF cells; ( E ) combinations on HeLa cells; ( F ) combinations on HFF cells. p -values (comparison to control): * p < 0.05, ** p < 0.001, *** p < 0.0001.

Article Snippet: The cytotoxicity was evaluated on using HeLa (ATCC ® CCL-2TM) and Human Foreskin Fibroblasts (HFF) (ATCC-SCRC-1041).

Techniques: Comparison, Control